Mannitol Dehydrogenase from Agaricus campestris

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Mannitol Dehydrogenase from Agaricus Campestris.

Enzymes capable of catalyzing n-mannitol formation from n-fructose or n-fructose 6-phosphate have been identified in various microorganisms. Substrate and coenzyme specificity have been established in only a few instances, but there appear to be enzymes, dependent upon nicotinamide adenine dinucleotide and upon nicotinamide adenine dinucleotide phosphate, which reduce fructose directly to manni...

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Pyranose Dehydrogenase from Agaricus campestris and Agaricus xanthoderma: Characterization and Applications in Carbohydrate Conversions

Pyranose dehydrogenase (PDH) is a flavin-dependent sugar oxidoreductase that is limited to a rather small group of litter-degrading basidiomycetes. The enzyme is unable to utilize oxygen as an electron acceptor, using substituted benzoquinones and (organo) metal ions instead. PDH displays a broad substrate specificity and intriguing variations in regioselectivity, depending on substrate, enzyme...

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Crystallization and preliminary crystallographic analysis of mannitol dehydrogenase (MtDH) from the common mushroom Agaricus bisporus.

Mannitol dehydrogenase (MtDH) is a key enzyme controlling the reductive synthesis of mannitol from fructose in the common mushroom Agaricus bisporus. A better understanding of the control of mannitol metabolism can be obtained by studying the structure of this enzyme. Here, the purification and crystallization of recombinant MtDH are reported. Crystals generally belonged to the space group C2, ...

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Studies on a hemagglutinin from the mushroom Agaricus campestris.

A phytohemagglutinin has been isolated in 80 to 90% purity from the mushroom Agaricus campestris. This protein had a sedimentation constant of 4.2 S and an electrophoretic mobility, at pH 8.6, remindful of the properties of a fast y-globulin. It was a nonspecific agglutinin reacting with erythrocytes and leukocytes from a number of species. The hemagglutination reaction was independent of tempe...

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Production of Oxalic Acid by a Strain of Agaricus campestris.

A strain of the common mushroom Agaricus campestris was grown on a mixture of composted sawdust and CaCO(3). On incubation for 47 days, the organism produced 20.5 g of oxalic acid per 100 g of initial dry compost solids.

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1963

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)75304-6